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Norovirus CE Digestive Test Kit RT QPCR Kit TaqMan Probe Four Enteroviruses

Categories Digestive Test Kit
Brand Name: Biokey
Model Number: BIK-QL-H007S
Certification: ISO 13485,CE
Place of Origin: Guangzhou China
MOQ: We can produce liquid and lyophilized kits
Price: USD
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
Delivery Time: Depending on order quantity
Packaging Details: Carton package
Package: 48 T/kit (single tube)
Specimen: Faeces
Specimen preservation: 2 weeks ar 4-8℃,3 months at -20± 5℃ and for a long time below -70℃.
Applicable: Micgene 244/244 IVD(BIO-KEY)
Detection limit: 500 copies/mL
CV: ≤5%
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Norovirus CE Digestive Test Kit RT QPCR Kit TaqMan Probe Four Enteroviruses

CE Mark Multiple four enteroviruses primers target Fluorescence probing RT qPCR kit


Intended use:

This kit is used for in vitro qualitative detection of Norovirus, Rotavirus, Adenovirus and Astrovirus nucleic acid RNA. It can be used for clinical auxiliary diagnosis of the etiological identification of suspected infected persons


Test Principle:


This kit uses fluorescent RT-PCR technology (TaqMan probe) to rapidly and accurately qualitatively detect the conserved regions of Norovirus/Rotavirus/Adenovirus/Astrovirus On the instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of Norovirus/Rotavirus/Adenovirus/Astrovirus through the change of fluorescent signal; the detection system contains a positive internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.


Product components:


The product components as following:


BIK-QL-H007S:

No.ComponentsAmount
11 (Norovirus and Rotavirus Mix (Lyophilized))24 tests/kit
22 (Adenovirus and Astrovirus Mix (Lyophilized))24 tests/kit
3Positive Control100 μL/tube
4Negative Control100 μL/tube
5RNase-free water1mL/tube
6Paraffin oil1.5mL/tube

Reaction Mix preparation:


Refer to the tables below to prepare the reaction mix:


Ⅰ. Norovirus and Rotavirus reaction mix:

1×volume required
Resuspended master mix14 μL
Primer-Probe Mix 11 μL
Total volume15 μL

Ⅱ. Adenovirus and Astrovirus reaction mix:

1×volume required
Resuspended master mix14 μL
Primer-Probe Mix 21 μL
Total volume15 μL

※Multiply the numbers according to the number of tests.


Data anlysis:


The following sample results are possible:

Ct valueResult Analysis
1#No CtNegative
2#≤38Positive
3#38~40Re-test; if it is still 38~40, report as 2#

Interpretation of test results:


(1)Norovirus and Rotavirus reaction:

FAM

(Norovirus)

VIC/HEX

(Rotavirus)

ROX

(IC)

Interpretation test results
1

-


-


-

Result invalid, test again
2+-+/-Norovirus(+)
3-++/-Rotavirus(+)
4+++/-Norovirus and Rotavirus(+)
5--+Negative(-)

(2)Adenovirus and Astrovirus reaction:

FAM

(Adenovirus)

VIC/HEX

(Astrovirus)

ROX

(IC)

Interpretation test results
1

-


-


-

Result invalid, test again
2+-+/-Adenovirus(+)
3-++/-Astrovirus(+)
4+++/-Adenovirus and Astrovirus(+)
5--+Negative(-)

NOTE: ‘+ ’ is positive, ‘- ’ is negative.


Operation details please check from IFU

Quality Norovirus CE Digestive Test Kit RT QPCR Kit TaqMan Probe Four Enteroviruses for sale
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