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Four Enteroviruses Human Diarrhea Detection Kit Multiple Rapid RT PCR Taqman probe

Categories Digestive Test Kit
Brand Name: Biokey
Model Number: BIK-QL-H007
Certification: ISO 13485,CE
Place of Origin: Guangzhou China
MOQ: We can produce liquid and lyophilized kits
Price: USD
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
Delivery Time: Depending on order quantity
Packaging Details: Carton package
Package: 96 T/kit (carton)
Specimen: Faeces
Specimen preservation: 2 weeks ar 4-8℃,3 months at -20± 5℃ and for a long time below -70℃.
Applicable: Micgene 244/244 IVD(BIO-KEY)
Detection limit: 500 copies/mL
CV: ≤5%
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Four Enteroviruses Human Diarrhea Detection Kit Multiple Rapid RT PCR Taqman probe

Four enteroviruses human diarrhea detection Multiple RT-PCR Fluorescence probing PCR test kit


Intended use:

This kit is used for in vitro qualitative detection of Norovirus, Rotavirus, Adenovirus and Astrovirus nucleic acid RNA. It can be used for clinical auxiliary diagnosis of the etiological identification of suspected infected persons


Test Principle:


This kit uses fluorescent RT-PCR technology (TaqMan probe) to rapidly and accurately qualitatively detect the conserved regions of Norovirus/Rotavirus/Adenovirus/Astrovirus On the instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of Norovirus/Rotavirus/Adenovirus/Astrovirus through the change of fluorescent signal; the detection system contains a positive internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.


Product components:


The product components as following:


1. BIK-QL-H007:

No.ComponentsAmount
1PCR Enzyme Mix (Lyophilized)96 tests/bottle
2

Primer-Probe Mix 1 (Norovirus and

Rotavirus)

50 μL/vial
3

Primer-Probe Mix 2 (Adenovirus

and Astrovirus)

50 μL/vial
4Enzyme Mix Buffer (5×)400 μL/vial
5Positive Control100 μL/tube
6Negative Control100 μL/tube
7RNase-free water1mL/tube
8Paraffin oil1.5mL/tube*2

Compatible instruments:


The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).


Interpretation of test results:


(1)Norovirus and Rotavirus reaction:

FAM

(Norovirus)

VIC/HEX

(Rotavirus)

ROX

(IC)

Interpretation test results
1

-


-


-

Result invalid, test again
2+-+/-Norovirus(+)
3-++/-Rotavirus(+)
4+++/-Norovirus and Rotavirus(+)
5--+Negative(-)

(2)Adenovirus and Astrovirus reaction:

FAM

(Adenovirus)

VIC/HEX

(Astrovirus)

ROX

(IC)

Interpretation test results
1

-


-


-

Result invalid, test again
2+-+/-Adenovirus(+)
3-++/-Astrovirus(+)
4+++/-Adenovirus and Astrovirus(+)
5--+Negative(-)

NOTE: ‘+ ’ is positive, ‘- ’ is negative.


Operation details please check from IFU

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