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Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized

Categories Digestive Test Kit
Brand Name: Biokey
Model Number: BIK-QL-H003S
Certification: ISO 13485,CE
Place of Origin: Guangzhou China
MOQ: We can produce liquid and lyophilized kits
Price: USD
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
Delivery Time: Depending on order quantity
Packaging Details: Carton package
Package: 48 T/kit (single tube)
Specimen: Human throat swabs, herpes fluid samples, stool samples, and anal swab samples.
Specimen preservation: be stored for 3 months at -20±5℃ and for a long time below -70℃
Applicable: Micgene 244/244 IVD(BIO-KEY)
Detection limit: 500 copies/mL
CV: ≤5%
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Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized

Real Time Diagnostics PCR Enterovirus 71 ( EV71 ) RT-PCR Detection Kit ( Lyophilized )


Intended use:

By using real time PCR systems, Enterovirus 71 real time PCR kit is used for the in vitro qualitative detection of Enterovirus 7. It can be used for the laboratory diagnosis and monitoring of the EV71 infection. The test results are only for clinical reference, not for case confirmation or exclusion.


Test Principle:


The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of EV71. The RT-PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of EV71 through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

Enterovirus 71 (EV71) RT-PCR Detection Kit (Lyophi...


Product components:


No.ComponentsBIK-QL-H003S
1PCR Enzyme Mix (Lyophilized)48 tests/kit
2EV71 Primer-Probe Mix/
3Enzyme Mix Buffer (5×)/
4Positive Control100 μL/tube
5Negative Control100 μL/tube
6RNase-free water1mL/tube
7Paraffin oil1.5mL/tube


Note: Do not mix the components from different batches for detection. The Positive Control of EV71 and internal control were constructed artificially, and they were not infectious.


Compatible instruments:


The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).


Reaction Mix Preparation:


Refer to one of the table below to prepare the reaction mix:


1×volume required

Resuspended master mix14 μL
EV71 Primer & probe1 μL
Total volume15 μL

Multiply the numbers according to the number of tests.


PCR amplification condition:


PCR amplification according to the following procedure:


StepCycleTemperatureTime
1150℃10 min
2194℃3 min

3


40

94℃10 s
60℃

30 s

(Collection of fluorescence)


Select the detection channels as following:

a) Select FAM channels to detect EV71.

b) Select HEX/VIC channel to detect internal control.


Reaction Mix Preparation:


Refer to one of the table below to prepare the reaction mix:

1×volume required
Resuspended master mix14 μL
EV71 Primer & probe1 μL
Total volume15 μL

Multiply the numbers according to the number of tests.


Product picture:



Operation details please check from IFU

Quality Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit  Lyophilized for sale
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