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HPV Aquaculture Test Kit Hepatopancreas Parvovirus Nucleic Acid Test Kit

Categories Aquaculture Test Kit
Brand Name: Biokey
Model Number: BIK-Q-W009
Certification: ISO 13485
Place of Origin: Guangzhou China
MOQ: We can produce liquid and lyophilized kits
Price: USD
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
Delivery Time: Depending on order quantity
Packaging Details: Carton package
Package: 24T/Box
Validity: 12 months
Storage: at -20±5℃, protected from light for 12 months
Applicable: Micgene 242 / 244 、ABI QuantStudio5
Mini limit: 10 copies/μL
CV: ≤5%
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HPV Aquaculture Test Kit Hepatopancreas Parvovirus Nucleic Acid Test Kit

Hepatopancreas Parvovirus ( HPV ) Nucleic Acid Magnetic Beads Fluorescent Probe PCR test kit



Intended use:


This kit is suitable for auxiliary diagnosis of hepatopancreas parvovirus (HPV) infection.

Principle:


This kit hepatopancreas parvovirus conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of hepatopancreas parvovirus was detected by fluorescence PCR apparatus in a completely closed reaction system.


Main components:

ComponentSpecification
PCR reaction liquid24 tubes
Positive control1 tube
Negative control1 tube
Note: components in different batches of kits should not be interchangeable.
Product picture:


Sample requirements:

1. Sample type

Edible prawn, machijiming prawn, vannamei and other susceptible prawn tissues

2. Sample collection

About 0.1g tissue samples were taken from prawn samples according to different sizes or infection stages. Among them, whole individuals were taken from larvae and larvae, head and chest of eyes were removed from juveniles under 3CM, gill area was taken from larger juveniles, and gill filaments or heart or lymphatic organs were taken from adult shrimp.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage.


PCR amplification and fluorescence detection (PCR detection area):


Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


StepCycle numberTemperatureTime
2195℃3min
34595℃10s
60℃30s collect fluorescent

FAM is selected for detection channels.


The results of interpretation:

ResultsCt Value
PositiveCt≤40
NegativeNo Ct value or Ct value=0
Gray area40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


Operation details please check from IFU!

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