Sign In | Join Free | My frbiz.com
frbiz.com
Products
Search by Category
Home > Other Chemical Reagents >

Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

Categories Aquaculture Test Kit
Brand Name: Biokey
Model Number: BIK-Q-W015
Certification: ISO 13485
Place of Origin: Guangzhou China
MOQ: We can produce liquid and lyophilized kits
Price: USD
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
Delivery Time: Depending on order quantity
Packaging Details: Carton package
Package: 24T/Box
Validity: 18 months
Storage: Store at room temperature, away from light
Applicable: Micgene 242 / 244 、ABI QuantStudio5
LoD: 10copies/μL
CV: ≤5%
  • Haven't found right suppliers
  • Our buyer assistants can help you find the most suitable, 100% reliable suppliers from China.
  • And this service is free of charge.
  • we have buyer assistants who speak English, French, Spanish......and we are ready to help you anytime!
Submit Buying Request
  • Product Details
  • Company Profile

Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

Enterocytozoon Acute hepatopancreatic necrosis syndrome Early mortality syndrome/Enterocytozoon hepatopenaei-18s(EMS/EHP-18S) Nuclear double nucleic acid test kit



Intended use:


This kit is suitable for the specific detection of Enterocytozoon hepatopenaei Acute hepatopancreatic necrosis syndrome Early mortality syndrome /Enterocytozoon hepatopenaei-18s (AHPND/EMS) and hepatoenteric cytiworm -18S (EHP-18S) in adult shrimp, prawn shrimp, shrimp seedlings, bait, processed aquatic products, etc.


Principle:


This kit is chosen prawn acute liver necrosis of pancreas disease and shrimp deeply cell worm - 18 s conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenchable group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of acute hepatopancreas necrosis and hepatoenteric cytiworm -18S nucleic acid of shrimp in the completely closed reaction system.


Main components:

ComponentSpecification
PCR reaction liquid24 tubes
Positive control1 tube
Negative control1 tube
Note: components in different batches of kits should not be interchangeable.


Sample requirements:

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products and other samples


2. Sample collection and transportation

Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.


3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.


Using Micgene 242/244/244IVD and ABI QuantStudio5:

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


StepCycle numberTemperatureTime
1195℃3min
24095℃10s
60℃30s collect fluorescent

FAM and VIC/HEX are selected for detection channels.


Result interpretation:


FAM channel (EMS)VIC/HEX channel(EHP-18S)Results judgment
1--The results were reported negative
2+-The results were reported EMS positive
3-+The results were reported EHP-18S positive
4++The results were reported EMS /EHP-18S positive

Note: "+" means positive, "-" means negative


The results of interpretation


ResultsCt Value
PositiveCt≤38
NegativeNo Ct value or Ct value=0
Gray area38<Ct≤40

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 40 is considered as


Operation details please check from IFU!

Quality Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit for sale
Send your message to this supplier
 
*From:
*To: Guangzhou BioKey Healthy Technology Co.Ltd
*Subject:
*Message:
Characters Remaining: (0/3000)
 
Inquiry Cart 0