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ASFV African Swine Fever Virus Porcine Test Kit PCR Taqman Polymerase Probe Test

Categories Porcine Test Kit
Brand Name: Biokey
Model Number: BIK-Q-S001
Certification: ISO13485
Place of Origin: Guangzhou China
MOQ: we produce both liquid and lyophilized kit
Supply Ability: 100,000 per day
Delivery Time: Depending on order quantity
Packaging Details: Carton package
Package: 48T/Box
Storage: Store away from light at -20±5℃
Validity: 12 months
Applicable: Micgene 242 / 244 、ABI QuantStudio5
Specimen: Oral swabs/blood/spleen/lymph nodes
CV: ≤5%
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ASFV African Swine Fever Virus Porcine Test Kit PCR Taqman Polymerase Probe Test

African swine fever virus Nucleic Acid Detection Kit

(PCR-Fluorescent Probe Method)



Intended use:

This kit is used for diagnosis, monitoring and epidemiological investigation of African swine fever (ASFV).

It is suitable for specific detection of African swine fever virus (ASFV) in pig oral swab, blood, spleen,

lymph node, kidney and other tissue samples.


Principle:

This kit to choose conservative African swine fever virus gene fragment design specific probe, the probe can

with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension,

the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down,

make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of ASF virus

was detected by fluorescence PCR in a completely closed reaction system.


Main components:


ComponentSpecification
PCR reaction liquid48 tubes
Positive control1 tube
Negative control1 tube
Note: components in different batches of kits should not be interchangeable.

The results of interpretation


ResultsCt Value
PositiveCt≤40
NegativeNo Ct value or Ct value=0
Gray area40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is

considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


Details please check IFU!


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