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Porcine Circovirus Type 2 Porcine Dna Test Kit PCR Nucleic Acid Test Micgene

Categories Porcine Test Kit
Brand Name: Biokey
Model Number: BIK-Q-S003
Certification: ISO 13485
Place of Origin: Guangzhou China
MOQ: We can produce liquid and lyophilized kits
Price: USD
Supply Ability: 100,000 per day
Delivery Time: Depending on order quantity
Packaging Details: Carton package
Package: 48T/Box
Specimen: Pig serum, feces, semen, viscera
Validity: 12 months
Storage: Store away from light at -20±5℃
Applicable: Micgene 242 / 244 、ABI QuantStudio5
CV: ≤5%
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Porcine Circovirus Type 2 Porcine Dna Test Kit PCR Nucleic Acid Test Micgene

Porcine circovirus type 2 (PCV2) Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)


Intended use:

This kit is used for diagnosis, monitoring and epidemiological investigation of porcine circovirus type 2, and is suitable for specific detection of porcine circovirus type 2 (PCV2) in pig serum, feces, semen, viscera and other tissue samples.


Principle:

This kit selection of porcine circovirus virus type 2 conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The porcine circovirus type 2 nucleic acid was detected by fluorescence PCR instrument in a completely closed reaction system.


Main components:


ComponentSpecification
PCR reaction liquid48 tubes
Positive control1 tube
Negative control1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:


1. Sample type:

Pig serum, feces, semen, viscera and other tissue samples

2. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.


The results of interpretation:

ResultsCt Value
PositiveCt≤40
NegativeNo Ct value or Ct value=0
Gray area40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


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