High Reproducibility Vitamin B5 (Pantothenic Acid) Test Kit 96 Test

Vitamin B5 (Pantothenic acid) Test Kit

Product Description

REAGEN^™Vitamin B5 Test Kit is quantitative analysis of total Vitamin B5 in food ,feed and drug.

The unique features of the kit are:

1. rapid, and cost-effective extraction methods,the same as other Vitamin.

2. The kit provide positive control and serial standards

3. High sensitivity (80μg/100g)

4. High recovery(80%-120%)

5. High reproducibility

Procedure Overview

REAGEN^™Sample and Vitamin B5 culture medium add to the 96-microwell which has the Lactobacillus plantarum, if the sample has Vitamin B5 the Lactobacillus plantarum will grow, after culture 37℃,44-48h, read the OD by microplate reader 610 - 630 nm (or540 - 550 nm),The sample of the turbidity compared with standard curve can get Vitamin B5 content.

Test protocol

All the reagents need to return to room temperature.shake the reagents before use. Don't pollute the kit’s reagents.

Add 2.5 mL sterile water to standard , cover cap and shake points dissolved with standard. The standard concentrate is 2400ug/100g. Filter the standard to a 4mL tube by 0.2um sterile filter.