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STD STI UP UU Real Time PCR Detection Kit 48 Tests/Kit Lyophilized

Categories STD STI Test Kit
Brand Name: Biokey
Model Number: BIK-QL-H0022S
Certification: CE
Place of Origin: Guangzhou China
MOQ: We can produce liquid and lyophilized kits
Price: USD
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
Delivery Time: Depending on order quantity
Packaging Details: Carton package
Package: 48 tests/kit
expiration date: 12 months
Storage: -20±5℃
Applicable: Micgene 244/244 IVD(BIO-KEY)
LoD: 1×1000 copies/ml
CV: ≤5%
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STD STI UP UU Real Time PCR Detection Kit 48 Tests/Kit Lyophilized

UP+UU real-time PCR detection kit (lyophilized)
--48 tests/kit


Intended use:

This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of Ureaplasma parvum and Ureaplasma urealyticum(UP+UU). It can be used for the laboratory diagnosis and monitoring of UP+UU infection. The test results are only for clinical reference, not for case confirmation or exclusion.

Principle:

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of UP+UU. The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of UP+UU through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

UP+UU product lyophilization pacakage:



Main components:

NO.Components

BIK-QL-H0022S

1

PCR Enzyme Mix(Lyophilized)

48 tests/kit

2Positive Control100 μL/tube
3Negative Control100 μL/tube
4RNase-free water1mL/tube
5

Paraffin oil

1.5mL/tube

Note:Do not mix the components from different batches for detection. The Positive Control of UP+UU and internal control were constructed artificially, and they were not infectious.


Specimen requirements:

1. Specimen type

Male urethral secretions or female cervical secretions.

2. Specimen Preservation

The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃


PCR Amplification condition:


PCR amplification according to the following procedures:

StepCycle numberTemperatureTime
1195℃3min
24095℃10s
60℃30s collect fluorescent

Instrument detection channel selection: FAM and HEX/VIC.

Quality control:


Negative Control: None of the FAM, VIC/HEX & Internal Control (ROX) channels have a Ct value or Ct > 40;


Positive Control: FAM, VIC/HEX & Internal Control (ROX) channels Ct ≤ 30;


The above two items should be met simultaneously in one experiment. Otherwise, the experiment is invalid and should be repeated.


Data analysis and interpretation:

FAM

(UP)

VIC/HEX

(UU)

ROX

(IC)

Interpretation test

results

1

-

-

-

Result invalid,test again

2+-+/-UP(+)
3-++/-UU(+)
4+++/-UP and UU(+)
5--+Negative(-)

NOTE: ‘+’ is positive, ‘-’ is negative.


Operation details please check from IFU!

Quality STD STI UP UU Real Time PCR Detection Kit 48 Tests/Kit Lyophilized for sale
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