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Bovine Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) ELISA Kit

Categories Bovine ELISA Kits
Place of Origin: China
Brand Name: DLdevelop
Certification: CE, ISO9001:2015
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
Model Number: DL-HSP90aB1-b
Assay length: 1-4.5Hours
Method: sandwich
Quality guarantee period: 12 month
Certificate: ISO9001:2015, CE
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    Bovine Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) ELISA Kit

    Bovine Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) ELISA Kit

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Bovine Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) ELISA Kit

    HSP90-BETA; HSP90B; HSPC2; HSPCB; HSP84; Heat Shock 90kD Protein 1,Beta; Heat shock 84 kDa

    Detection range:1.56-100ng/mL
    Quality guarantee period:for 12 months
    Catalog number:DL-HSP90aB1-b (traditional)DLR-HSP90aB1-b (ready-to-use)
    Assay length1-4.5Hours1-3.5Hours
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual

    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HSP90aB1 serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120μLDetection Solution A 1×12mL
    Detection Reagent B 1×120μLDetection Solution B 1×12mL
    TMB Substrate 1×9mLTMB Substrate 1×9mL
    Stop Solution 1×6mLStop Solution 1×6mL
    Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
    Instruction manual 1Instruction manual 1
    Test principle

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP90aB1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HSP90aB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

    After TMB substrate solution is added, only those wells that contain HSP90aB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm.

    The concentration of HSP90aB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.


    Matrices listed below were spiked with certain level of recombinant HSP90aB1 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP90aB1 in samples.

    MatrixRecovery range (%)Average(%)
    EDTA plasma(n=5)81-10191
    heparin plasma(n=5)80-8984

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP90aB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    EDTA plasma(n=5)88-101%86-95%90-102%80-93%
    heparin plasma(n=5)80-91%82-90%95-104%79-95%

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